Educational Information
What is the principle of Beckman Access 2 Immuno analyzer?
The Beckman Access 2 is an immunoassay analyzer used in clinical laboratories for the quantitative determination of various analytes, including hormones, proteins, and infectious disease markers. The principle behind the Beckman Access 2 involves a few key steps:
1. Immunoassay Technique: The Access 2 primarily employs a two-site sandwich immunoassay method. This technique involves the use of specific antibodies that bind to the target analyte in a sample (such as serum or plasma).
2. Sample Introduction: The sample is introduced into the system, where it mixes with specific reagents that contain the antibodies and other necessary components for the assay.
3. Binding and Reaction: The analyte in the sample binds to the antibodies, forming a complex. A second set of labeled antibodies (often linked to a detectable marker) binds to another site on the analyte, forming a sandwich complex.
4. Detection: The Beckman Access 2 uses chemiluminescent detection, where the labeled antibodies generate light in response to a chemical reaction. The intensity of the light produced is directly proportional to the concentration of the analyte in the sample.
5. Quantification: The system measures the emitted light, and the results are quantified using calibration curves established with known standards. This allows for the calculation of the concentration of the target analyte in the sample.
6. Automation and Results: The Beckman Access 2 is designed for high throughput, providing automated sample processing, result generation, and quality control to enhance efficiency in clinical settings.
Overall, the Beckman Access 2 combines sophisticated immunoassay techniques with advanced detection methods to provide accurate and reliable results for various clinical tests.